Direct Determination of Saccharin and Acesulfame-K

NATALIA E. LLAMAS, MARÍA S. DI NEZIO, MIRIAM E. PALOMEQUE, BEATRIZ S. FERNÁNDEZ BAND

Food Analytical Methods

Springer

Lugar: New York; Año: 2008 vol. 1 p. 43 - 48

1936-9751

Abstract A simple and rapid analytical procedure for determination of saccharin (SAC) and acesulfame-K (ACK) based on UV-vis measurements and partial least squares (PLS) was proposed. Thus, an experimental design at levels 2 and 15 mg l-1 for SAC and 2 and 20 mg l-1 for AC-K was applied. Because real samples usually contain SAC and AC-K combined with aspartame (ASP), this interference was also included in the model. The procedure was successfully applied for SAC and AC-K simultaneous determination in sweeteners and fruit juice powders, without any separation step to remove ASP. The results were validated by using spiked samples, and the obtained recoveries were satisfactory. A simple and rapid analytical procedure for determination of saccharin (SAC) and acesulfame-K (ACK) based on UV-vis measurements and partial least squares (PLS) was proposed. Thus, an experimental design at levels 2 and 15 mg l-1 for SAC and 2 and 20 mg l-1 for AC-K was applied. Because real samples usually contain SAC and AC-K combined with aspartame (ASP), this interference was also included in the model. The procedure was successfully applied for SAC and AC-K simultaneous determination in sweeteners and fruit juice powders, without any separation step to remove ASP. The results were validated by using spiked samples, and the obtained recoveries were satisfactory. -1 for SAC and 2 and 20 mg l-1 for AC-K was applied. Because real samples usually contain SAC and AC-K combined with aspartame (ASP), this interference was also included in the model. The procedure was successfully applied for SAC and AC-K simultaneous determination in sweeteners and fruit juice powders, without any separation step to remove ASP. The results were validated by using spiked samples, and the obtained recoveries were satisfactory.