EFFECT OF OXIDATIVE STRESS ON ADIPOCYTE DIFFERENTIATION

BLAZQUEZ, ANA; PIWIEN-PILIPIUK, G; SALVADOR, G.A.; URANGA, R.

Buenos Aires

Congreso; LIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)- Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedades de Biociencias Argentina

(906) EFFECT OF OXIDATIVE STRESS ON ADIPOCYTEDIFFERENTIATIONAna Catalina Blazquez (1, 2), Graciela Piwien-Pilipuk (3),Gabriela Alejandra Salvador (1, 2), Romina María Uranga(1, 2)(1) Instituto de Investigaciones Bioquímicas de Bahía Blanca,Universidad Nacional del Sur (UNS)-CONICET, BahíaBlanca, Argentina, (2) Departamento de Biología, Bioquímicay Farmacia, UNS, Bahía Blanca, Argentina, (3) Institutode Biología y Medicina Experimental (IByME)-CONICET,Buenos Aires, Argentina.Oxidative stress (OS) is a major characteristic of adipose tissue inobese patients and its role in adipose hyperplasia/hypertrophy hasnot been elucidated. The aim of our study was to analyze the effectof OS during adipogenesis as well on differentiated adipocytes. Forthis purpose, we worked with preadipocytes 3T3-L1 and menadione,a synthetic form of vitamin K known to generate intracellular oxygenspecies. Two different models were used: the ?chronic?, in which OS(5, 10 y 15 µM menadione) was present during the whole process of3T3-L1 differentiation, and the ?acute?, in which differentiated adipocyteswere exposed to OS (20 y 50 µM menadione) for 5 h.Adipogenesis, evaluated by Oil Red O staining as well as by theexpression of adipogenic markers (PPARγ, C/EBPα, FAS, FABP4)by Western blot, was observed to be decreased in the chronic modelin a menadione concentration-dependent manner (p