LIPID DROPLETS AS AN EARLY MARKER OF α-SYNUCLEIN-INDUCED NEURODEGENERATION

ALZA, NATALIA P.; CONDE, MELISA A.; SCODELARO BILBAO, P; SALVADOR GABRIELA

Buenos Aires

Congreso; . LIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)- Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedades de Biociencias Argentina

(447) LIPID DROPLETS AS AN EARLY MARKER OFα-SYNUCLEIN-INDUCED NEURODEGENERATIONNatalia Alza (1), Melisa Conde (2), Paola Scodelaro Bilbao(3), Gabriela Salvador (2)(1) Dpto. Qca.-UNS; INIBIBB-CONICET. (2) Dpto. Biol.,Bioq. y Farm.-UNS; INIBIBB-CONICET. (3) Dpto. Biol., Bioq.y Farm.-UNS; INIBIBB-CONICET, CERZOS-CONICET.Pathological accumulation of α-synuclein (α-syn), a cytosolic proteinhighly expressed in the central nervous system, is a hallmarkof Parkinson?s disease. α-syn has the ability to bind lipids, althoughits involvement in lipid metabolism remains unknown. In previouswork, we reported that the overexpression of A53T α-syn in dopaminergicneurons increased the cellular content of neutral lipids andfatty acids (SAIB 2014-2015). The aim of the present study was tocharacterize the role of α-syn in neuronal lipid metabolism. For thispurpose, we worked with a human neuroblastoma cell line, IMR32,that stably express the wild type form of α-syn (WT α-syn). Weobserved an increase in triacylglycerides (TAG) and cholesterolcontent in WT α-syn neurons when compared with control cells(pcDNA3) (**p