INVESTIGADORES
SALVADOR Gabriela Alejandra
congresos y reuniones científicas
Título:
Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
Autor/es:
SANCHEZ CAMPOS, S; RODRIGUEZ DIEZ, G; URANGA, R.; SALVADOR, G.
Lugar:
Mendoza
Reunión:
Congreso; SAIB; 2012
Institución organizadora:
SAIB
Resumen:
Metal-induced
oxidative stress activates different lipid signaling pathways in dopaminergic
neurons.
Sánchez Campos, Sofía; Rodríguez Diez,
Guadalupe; Uranga, Romina; Salvador, Gabriela.
The
characterization of the mechanisms mediating the effects of metal-induced
oxidative stress on neuronal dysfunction and death is central in the
understanding of the pathology of several neurodegenerative disorders such as
Parkinsons disease. In this work, we characterized the cellular responses that
operate in dopaminergic neurons (N27 cells) exposed to an overload of
transition metals such as iron (Fe, 1 mM), copper (Cu, 10 and 50 μM) or their
combination for 24 hs. Under these experimental conditions, reactive oxygen species measured by fluorescence microscopy, and lipid
peroxidation levels increased as a function of metal concentration. Maximum
levels of lipid peroxides were observed in the presence of Fe + Cu. Cell
viability, determined by MTT reduction, strongly decreased in the presence of Cu
and with the combination of both metals. Under these experimental conditions, an
increase in the levels of Akt phosphorylation in Ser-473 was observed. Bcl-2
expression showed the same profile that Akt phosphorylation. In addition, the
expression and the activation of the secretory and cytosolic isoforms of phospholipase
A2 (PLA2) were differentially affected by metal overload. Our results
demonstrate that phospholipid deacylation processes catalyzed by PLA2s and PI3K
activation are involved in the response of dopaminergic neurons to
metal-induced oxidative stress.