Neutrophil Migration to Lymph Nodes through Lymph and Blood in an Immune Inflammation Model.

CAROLINA GORLINO; ROMINA P. RANOCCHIA; GABRIEL MORÓN; BELKYS MALETTO; MARÍA C. PISTORESI-PALENCIA

Viña del Mar, Chile

Congreso; IX Congreso de la Asociación Latinoamericana de Inmunología; 2009

Asoc. Latinoamericana de Inmunología

We previously showed that when OVA-FITC was injected into the footpad of OVA/CFA immunized mice, the mainOVA-FITC+ cells recruited in draining popliteal lymph nodes were neutrophils and this influx was dependent on anantigen-specific response. On the basis of these findings, we investigated if neutrophils can enter into lymph nodestrough afferent lymphatics and blood. Immunofluorescence of lymph node sections of OVA/CFA-immunized miceinjected with OVA-FITC showed the presence of neutrophils OVA-FITC+ in the lumen of lymphatic vessels (LYVE-1+)and in the high endothelial venules (MECA-79+). Bone marrow neutrophils incubated with or without immunecomplexes (anti-OVA rabbit sera plus OVA) were labeled with CFSE and injected in the footpad or vein of OVA/CFAimmunizedmice. Neutrophils were only found in draining popliteal lymph nodes of mice injected with neutrophilsincubated with immune complexes (p<0.05) and not in mice injected with neutrophils without immune complexes. Inaddition, this migration was dependent on inflammatory condition (p<0.01 non-immunized mice vs OVA/CFAimmunized mice). In conclusion, these data provide new evidence about the neutrophil trafficking from skin (by lymphvessels) or from blood into lymph nodes in an immune inflammation model