RECRUITMENT OF IMMUNOCOMPLEX-ACTIVATED NEUTROPHILS MODULATE T CELL RESPONSE IN LYMPH NODES

SOFÍA CASTELL; FLORENCIA HARMAN; MORON VG; BELKYS A. MALETTO; MARÍA C PISTORESI- PALENCIA

Mar del Plata

Congreso; LXIV Reunión de la Sociedad Argentina de Inmunología; 2016

Sociedad Argentina de Inmunolog

&amp;lt;!-- /* Font Definitions */ @font-face{font-family:Helvetica;panose-1:0 0 0 0 0 0 0 0 0 0;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1342208091 0 0 415 0;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-alt:Calibri;mso-font-charset:0;mso-generic-font-family:swiss;mso-font-pitch:variable;mso-font-signature:-536859905 -1073732485 9 0 511 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-TRAD;mso-fareast-language:EN-US;}p.MsoBodyText, li.MsoBodyText, div.MsoBodyText{mso-style-priority:1;mso-style-unhide:no;mso-style-qformat:yes;mso-style-link:"Texto independiente Car";margin-top:0cm;margin-right:0cm;margin-bottom:0cm;margin-left:2.0pt;margin-bottom:.0001pt;mso-pagination:widow-orphan;mso-layout-grid-align:none;text-autospace:none;font-size:7.5pt;font-family:Helvetica;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-bidi-font-family:Helvetica;mso-fareast-language:EN-US;}span.TextoindependienteCar{mso-style-name:"Texto independiente Car";mso-style-priority:1;mso-style-unhide:no;mso-style-locked:yes;mso-style-link:"Texto independiente";mso-ansi-font-size:7.5pt;mso-bidi-font-size:7.5pt;font-family:Helvetica;mso-ascii-font-family:Helvetica;mso-hansi-font-family:Helvetica;mso-bidi-font-family:Helvetica;mso-ansi-language:ES-AR;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-TRAD;mso-fareast-language:EN-US;}@page WordSection1{size:20.0cm 794.0pt;margin:0cm 57.0pt 0cm 80.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}--&amp;gt;Previouslywe demonstrated that immunocomplexes (IC) generated by injecting OVA in thefootpad of immunized mice that have anti-OVA antibodies, induce neutrophils-OVA+ migration to draining popliteal lymph nodes (D-poLNs). In the presentstudy we evalu- ate the influence of IC-activated neutrophils on T-cellresponse in lymph nodes. C57BL/6 mice were immunized with OVA emulsified inFreund´s complete adjuvant and 15 days later boosted with OVA emulsified inFreund´s incomplete adjuvant. Ten days after last immunization they wereinjected with OVA-FITC in the footpad and D-poLNs were obtained at differenttimes (between 3 and 48 h); saline solution was injected on footpadcorresponding to control non-draining popliteal lymph nodes (ND-poLNs). Thedif- ferent cell populations in the lymph nodes were characterized by flowcytometry. Three hours after footpad injection, an increased number of OVA-FITC+ Ly6Ghi neutrophils was observed in D-poLNscompared to ND-poLNs (p<0.05). The highest levels of OVA-FITC+ neutrophils were detected 6 h after OVA injection (p<0.001), at 12 hthe number of neutrophils decreased to basal levels. However, total number ofD-poLN cells increase after 24 h of OVA injec- tion (p<0.01). At24 h weobserved an increase of dendritc cells (CD11c+) inD-poLNs compared to ND-poLNs (p<0.01). At 48 h we observed an increase ofCD4 T cells (p<0.01), particularly effector CD4 T-cells (CD44+ CD62L-) (p<0.01) with respect to ND-poLNs.Besides, we observed higher percentage of Ki67+ CD4T-cells in immunized mice 24 h after OVA injection (p<0.001), indicatingthat CD4 T-cells have higher levels of proliferation after recruitment ofIC-activated neutrophils. These results suggest that neutrophils recruitment indraining popliteal lymph nodes could be modulating the T-cell response.