Host type I IFN signals are required for the antitumor immune response elicited by the therapeutically administered TLR3 ligand Poly AU.

DAVID ANDRÉS NOCERA; NICOLÁS NÚÑEZ; GERARDO GATTI; EMILIANO ROSELLI; PAULA ARAYA; ESTEFANIA ZACCA; GABRIEL MORON; MARIANA MACCIONI

Los Cocos, Córdoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de inmunologia

Introduction: The use of viral double-stranded RNA (dsRNA) mimetics in cancer immunotherapy has been explored for several decades with the idea of enhancing innate and adaptive immune responses and altering the tumor microenvironment. Polyadenylic?polyuridylic acid (pAU) is a synthetic analog of viral dsRNA recognized mainly by Toll like receptor 3 (TLR3). The main goal of this work was to analyze the effect of intratumorally (i.t) administered pAU on the specific immune response against tumor antigens in ifnar deficient mice. Material and Methods: B16 murine melanoma cells expressing Ovalbumin (OVA) were inoculated s.c. in wild-type and ifnar-/- mice. When tumors reached a measurable size (~day 12 p.i), a group of mice from each strain was intratumorally treated with 50 µg of pAU (n=10, 4 doses every two days). Results: pAU-treated wild-type mice showed a significantly reduced tumor growth as well as a longer survival compared to controls (p