Inorganic mercury in mammary cells: viability, metal uptake but efflux?

MANIERO, MARIÁNGELES ÁVILA; GUERRERO-GIMENEZ, MARTIN E.; FANELLI, MARIEL A.; WUILLOUD, RODOLFO G.

BIOMETALS

SPRINGER

Lugar: Berlin; Año: 2017

0966-0844

The viability, cellular uptake and subcellulardistribution of heavy metal Hg, were determinedin human mammary cell lines (MCF-7, MDA-MB-231and MCF-10A). It was observed that Hg had thecapacity of being excluded from the cells with adifferent type of possible transporters. MCF-7 cellsshowed the lowest viability, while the other two celllines were much more resistant to Hg treatments. Theintracellular concentration of Hg was higher at lowerexposure times in MCF-10A cells and MCF-7 cells;but as the time was increased only MDA-MB-231showed the capacity to continue introducing the metal.In MCF-7 and MCF-10A cells the subcellular distributionof Hg was higher in cytosolic fraction thannucleus and membrane, but MDA-MB-231 showedmembrane and nucleus fraction as the enriched one.The analysis of RNA-seq about the genes or family ofgenes that encode proteins which are related tocytotoxicity of Hg evidenced that MCF-10A cellsand MCF-7 cells could have an active transport toefflux the metal. On the contrary, in MDA-MB-231 nogenes that could encode active transporters have beenfound