INVESTIGADORES
SALA Adriana Andrea
artículos
Título:
The 20002001 GEPISFG Collaborative Exercise on mtDNA:
Autor/es:
PRIETO, MONTESINO, SALAS, ALOSNSO, ALBARRAN, ALVAREZ, CRESPILLO, CORACH, SALA ET AL
Revista:
FORENSIC SCIENCE INTERNATIONAL.
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Ireland; Año: 2003 vol. 134 p. 46 - 53
ISSN:
0379-0738
Resumen:
We report the results of Spanish and Portuguese working group (GEP) of International Society of Forensic Genetics (ISFG)
Collaborative Exercise 20012002 on mitochondrial DNA (mtDNA) analysis. 64 laboratories from Spain, Portugal and several
Latin-American countries participated in this quality control exercise. Five samples were sent to the participating laboratories,
four blood stains (M1M4) and a sample (M5) consisting of two hair shaft fragments. M4 was non-human (Felis catus) in origin;
therefore, the capacity of the labs to identify the biological source of this sample was an integral part of the exercise. Some labs
detected the non-human origin of M4 by carrying out immuno-diffussion techniques using antihuman serum, whereas others
identified the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of
mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1M3) improved in relation to
the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous
results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not
the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.
As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for
the labs performing mtDNA analysis in forensic casework.2002 on mitochondrial DNA (mtDNA) analysis. 64 laboratories from Spain, Portugal and several
Latin-American countries participated in this quality control exercise. Five samples were sent to the participating laboratories,
four blood stains (M1M4) and a sample (M5) consisting of two hair shaft fragments. M4 was non-human (Felis catus) in origin;
therefore, the capacity of the labs to identify the biological source of this sample was an integral part of the exercise. Some labs
detected the non-human origin of M4 by carrying out immuno-diffussion techniques using antihuman serum, whereas others
identified the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of
mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1M3) improved in relation to
the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous
results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not
the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.
As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for
the labs performing mtDNA analysis in forensic casework.M4) and a sample (M5) consisting of two hair shaft fragments. M4 was non-human (Felis catus) in origin;
therefore, the capacity of the labs to identify the biological source of this sample was an integral part of the exercise. Some labs
detected the non-human origin of M4 by carrying out immuno-diffussion techniques using antihuman serum, whereas others
identified the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of
mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1M3) improved in relation to
the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous
results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not
the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.
As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for
the labs performing mtDNA analysis in forensic casework.fied the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of
mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1M3) improved in relation to
the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous
results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not
the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.
As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for
the labs performing mtDNA analysis in forensic casework.M3) improved in relation to
the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous
results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not
the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.
As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for
the labs performing mtDNA analysis in forensic casework.ficiency was not
the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.
As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for
the labs performing mtDNA analysis in forensic casework.fic hair samples and the degradation.
As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for
the labs performing mtDNA analysis in forensic casework.ficiency testing as part of the accreditation procedure for
the labs performing mtDNA analysis in forensic casework.