INVESTIGADORES
BARRA Jose Luis
artículos
Título:
Histone H1 is dispensable for Methylation-associated gene silencing in Ascobolus and essential for long life-span.
Autor/es:
JOSÉ LUIS BARRA; L. RHOUNIM,; G. FAUGERON,; J.-L. ROSSIGNOL,
Revista:
MOLECULAR AND CELLULAR BIOLOGY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Año: 2000 vol. 20 p. 61 - 69
ISSN:
0270-7306
Resumen:
Ascobolus immersus. The deduced peptide sequence presents the characteristic three-domain structure of
metazoan linker histones, with a central globular region, an N-terminal tail, and a long positively charged
C-terminal tail. By constructing an artificial duplication of this gene, named H1, it was possible to methylate
and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of
the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing,
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of
the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing,
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
metazoan linker histones, with a central globular region, an N-terminal tail, and a long positively charged
C-terminal tail. By constructing an artificial duplication of this gene, named H1, it was possible to methylate
and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of
the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing,
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of
the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing,
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
. The deduced peptide sequence presents the characteristic three-domain structure of
metazoan linker histones, with a central globular region, an N-terminal tail, and a long positively charged
C-terminal tail. By constructing an artificial duplication of this gene, named H1, it was possible to methylate
and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of
the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing,
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of
the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing,
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
H1, it was possible to methylate
and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of
the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing,
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing,
underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type
strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA
was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These
features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
Ascobolus H1 histone is a ubiquitous component of chromatin which plays no
role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through
sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination,
they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.
Ascobolus H1 histone is necessary for long
life span. This constitutes the first observation of a physiologically important phenotype associated with the
loss of H1.