Glycine Betaine Transmethylase Mutant of Pseudomonas aeruginosa.

L. SERRA, A.; F. MARISCOTTI, JAVIER; JOSÉ LUIS BARRA; I. LUCHESI, G.; C. DOMENECH,; T. LISA., A.

JOURNAL OF BACTERIOLOGY

AMER SOC MICROBIOLOGY

Año: 2002 vol. 184 p. 4301 - 4303

0021-9193

The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.