Effect of Lactobacillus fermentum CRL1446 on immune-metabolic parameters and intestinal microbiota composition in obesity in high-fat diet fed mice

GAUFFIN-CANO, MARÍA PAOLA; FABERSANI, EMANUEL; MARQUEZ, ANTONELA; ROSS, ROMINA; ABEIJÓN-MUKDSI, CLAUDIA; MEDINA, ROXANA.

Buenos Aires

Congreso; IUNS21st International Congress of Nutrition; 2017

Dietary strategies, including the use ofprobiotics as preventive agents that handle the intestinal microbiota and/ormodulate the function of adipose tissue, would be suitable tools for theprevention or treatment of different conditions associated with obesity. Inthis context, our aim was to evaluate the effect of Lactobacillus fermentumCRL1446 (CRL1446) on metabolic and immunological parameters and on thecomposition of intestinal microbiota in a model of obesity induced by ahigh-fat diet (HFD).Methods: Balb/c mice were separated into thefollowing groups: 1) SD: Standard diet, 2) HFD: High-fat diet, 3) HFD+CRL1446:mice receiving a HFD and a daily dose of 1 x 108 CFU of L. fermentum CRL1446(CRL1446) in the drinking water. The dietary intervention was maintained for 6weeks. Weight and food intake were monitored daily. It were determined at 20and 45 days of experimental period: fat tissue weight (histologic studies),metabolic parameters (triglyceride (TG), total cholesterol (TC), glucose (Glu)and leptin (Lep)), seric immunologic parameters (TNF-alpha, IL-6, IL10 andMCP-1), macrophage and adipocyte functionality (ex vivo studies) and the gutmicrobiota composition (evaluated by high-throughput sequencing of 16S rRNAgene amplicons).Results: CRL1446 strain induces a decrease inbody and adipose tissue weights and the size of adipocytes. A decrease inplasmatic glucose, cholesterol, triglycerides, leptin, TNF-alpha and IL-6levels and an increase of IL-10 were observed. Ex vivo studies showed a minorsecretion of leptin by adipocytes. In macrophages, CRL1446 induced lower levelsof MCP-1, TNF-alpha, IL-6 and higher levels of IL-10. Regarding intestinalmicrobiota, an increase in Lactobacillus and a decrease inFirmicutes/Bacteroidetes (F/B) index were observed.Conclusions: The supplementation with L. fermentum CRL1446 producechanges in intestinal microbiota structure and metabolic and immunologicparameters. CRL1446 could potentially be a probiotic strain useful to correctthe immuno-metabolic alteration