BIFIDOBACTERIUM ENZYMES INVOLVED IN LIPIDS METABOLISM: IMPACT ON THE DEVELOPMENT OF FUNCTIONAL FOODS

TERAN, V; MEDINA, R; VAN NIUEWENHOVE, C

Mar del Plata

Jornada; LI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2015

LI ReuSociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)

Fewstudies showed the influence of bifidobacteria over lipids derived from milkproducts, especially fatty acids (FA) hydrolysis by esterase and lipasesenzymes and production of CLA and CLNA by isomerases with health promoting properties.The aim of this work was to determine enzymes activities by 3 bifidobacteria:INL2, ZL1228 and LM7a, isolated from breast milk and cultured in MRS?cys at 37°C, 48 h under anaerobic conditions. Esterase activity was measured in cell freeextracts by PAGE using acrylamide gels at 12% without SDS and incubated inpresence of a-naphtyl derivates from FA (C2?C12) and FastRed TR as developer. To evaluate bioactive lipids production by GC bacteriawere cultured in MRS-cys and skim milk 10% with LA and LNA. The highest levelof esterase activity was detected against a-naphtylacetate, propionate y butyrate. Low levels were obtained in presence of C10 and12 for LM7a and ZL1228. Electrophoretic zymograms showed 4 esterase enzymeswith different electrophoretic mobilities and substrate specificity. No lipaseactivity was detected. All strains produce CLA in MRS-cys and skim milk. INL2 producedthe highest levels of CLNA in MRS-cys while ZL1228 resulted the best producerin skim milk. This study shows bifidobacteria with esterase and isomeraseactivity, both important for the development of functional foods with desirableorganoleptic characteristics.