Specific Strains of Lactic Acid Bacteria Differentially Modulate the Profile of Adipokines In Vitro

RUSSO, M., FABERSANI, E., ABEIJÓN MUKDSI, M.C., GONZÁLEZ, S. GAUFFIN CANO, P. MEDINA, R.B; ABEIJÓN MUKDSI, M.C.; ROSS, R.; MEDINA, R.; GONZÁLEZ,S.; GAUFFIN CANO, P

FRONTIERS IN INMUNOLOGY

Frontiers

Lugar: Lausanne ; Año: 2017

Obesityinduces local/systemic inflammation accompanied by increases in macrophage infiltrationinto adipose tissue and production of inflammatory cytokines, chemokines, andhormones. Previous studies have shown that probiotics could improve theintestinal dysbiosis induced by metabolic diseases such as obesity, diabetes,and metabolic syndrome.Microorganismscould (directly or indirectly) affect adipokine levels due to their capacity toinduce translocation of several intestinal microbial antigens into systemic circulation,which could lead to metabolic endotoxemia or produce immunomodulation indifferent organs. The aim of the present study was to select non-inflammatorylactic acid bacteria (LAB) strains with the capacity to modulate adipokinesecretion by the adipose tissue. We wish to elucidate the role of potentialprobiotic strains in the regulation of the cross talking between immune cellssuch as macrophages and adipose cells. Mouse macrophage cell line RAW 264.7 wasused for evaluating the ability of 14 LAB strains to induce cytokineproduction. The LAB strains were chosen based on their previously studiedbeneficial properties in health. Then, in murine adipocyte culture and macrophage?adipocytecoculture, we determined the ability of these strains to induce cytokines andleptin secretion. Tumor necrosis factor alpha, interleukin 6 (IL-6), IL-10, monocytechemoattractant protein-1, and leptin levels were measured in cellsupernatants.We also performed the detection and quantification ofleptin receptor (Ob-Rb) expression in macrophage cell lines stimulated by theseLAB strains. Differential secretion profile of cytokines in macrophage cellsinduced by LAB strains was observed. Also,the levels of Ob-Rb expressiondiverged among different LAB strains. In LAB-stimulated coculture cells(adipocytes and macrophages), we observed differential production of leptin andcytokines. Furthermore, we detected lower production levels in single culture thancocultured cells. The principal component analysis showed an associationbetween the four clusters of strains established according to theirinflammatory profiles and leptin adipocyte production and leptin receptorexpression in macrophages. We conclude that coculture is the most appropriatesystem for selecting strains with the ability to modulate adipokine secretion.The use of microorganisms with low and medium inflammatory properties andability to modulate leptin levels could be a strategy for the treatment of somemetabolic diseases associated with dysregulation of immune response