Activation of PPARã regulates Sertoli cell nutritional function

GORGA AGOSTINA; RINDONE GUSTAVO; REGUEIRA MARIANA; PELLIZZARI ELIANA HERMINIA; CAMBEROS MARíA DEL CARMEN; CIGORRAGA SELVA BEATRIZ; RIERA MARíA FERNANDA; GALARDO MARíA NOEL; MERONI SILVINA BEATRIZ

Mar del Plata

Congreso; LXI Reunion Anual de la Sociedad Argentina de Investigación Clínica (SAIC),; 2016

Sociedad Argentina de Investigación Clínica (SAIC),

Sertoli cells (SC) provide the structural and nutritional support for germ cell development. Studies on SC glucose metabolism have shown that this cell type actively metabolizes glucose and converts it to lactate, which is an important source of energy for germ cells. Furthermore, it has been demonstrated that SC can oxidize FA and it is assumed that this metabolic process fulfills the energy requirements of SC. FA are stored as triacylglycerides (TAG) within lipid droplets (LD). Recently, we have demonstrated that the transcription factor PPARã participates in the expression of genes involved in the regulation of lipid storage as TAG in LD. In this metabolic context, the simultaneous regulation of FA storage and lactate production may be relevant to seminiferous tubule physiology. The aim of this work was to study the participation of PPARã activation in the regulation of lactate production in SC. Cultures of SC obtained from 20-day-old rats were incubated for different periods of time without (basal, B) or with Rosiglitazone ?RSG, pharmacological activator of PPARã. Results are expressed as mean±SD of three independent experiments obtained in 48h incubations with 10µM RSG (*p