Activation of PPARa and PPARb/d regulates Sertoli cell metabolism

REGUEIRA MARIANA; RIERA MARÍA FERNANDA; ARTAGAVEYTIA SILVANA; GALARDO MARÍA NOEL LUJÁN; PELLIZZARI ELIANA HERMINIA; CIGORRAGA SELVA BEATRIZ; MERONI SILVINA BEATRIZ

HOUSTON

Congreso; 94th ANNUAL MEETING ENDO 2012; 2012

ENDOCRINE SOCIETY

Sertoli cells (SC) provide the structural and nutritional support for germ cell development. Studies on SC glucose metabolism have shown that this cell type actively metabolizes glucose and converts it to lactate, which is the major source of energy for germ cells. On the other hand, it has been postulated that SC uses fatty acids (FA) as a source of energy. The transcription factors PPARα and PPARβ/δ ?members of the PPARs nuclear receptor family? participate in the expression of genes involved in FA metabolism in many cells types. The regulation of FA metabolism in SC has not been analyzed in detail. The aim of this work was to study the participation of PPARα and PPARβ/δ activation in the regulation of genes involved in FA metabolism and in lactate production in SC. Cultures of SC obtained from 20-day-old rats were incubated for different periods of time without (basal) or with variable doses of WY14643 (WY) or GW0742 (GW) ?pharmacological activators of PPARα and PPARβ/δ respectively. Carnitine palmitoyltransferase 1 (CPT1) mRNA levels were detected by Northern Blot and long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases (LCAD, MCAD) and the fatty acid transporter CD36 (FAT/CD36) mRNA levels were analyzed by RQPCR. Results expressed as fold-increase in mRNA levels combining three independent experiments (mean±SD), obtained in 48h incubations with 10 µM WY and 5 µM GW are shown; CPT1 WY:2.3±0.6*; GW: 3.3±0.2*; LCAD WY:1.9±0.4*; GW:2.8±0.3*; MCAD WY: 1.9±0.3*; GW:1.8±0.4* and FAT/CD36 WY:2.5±0.6*, GW:2.7±0.5* (*p