Effect of lactate dehydrogenase (LDH) activity suppression on the regulation of Sertoli cell (SC) proliferation by FSH.

CENTOLA, C.L.; DASSO, M.E.; SORIA D; RIERA, M.F.; MERONI, S.B.; GALARDO, M.N.

MAR DEL PLATA

Congreso; LXIi Reunion Anual de la Sociedad Argentina de Investigacion Clinica; 2022

Sociedad Argentina de Investigacion Clinica

The final number of SC reached during the proliferative periods determines sperm production capacity in adulthood. It is well known that FSH is the major SC mitogen exerting its action by activation, at least in part, of mTORC1/p70S6K pathway. mTORC1 regulates a wide range of cellular functions, including glycolytic metabolism and cell proliferation. Moreover, new evidence evinces that glycolytic flux is involved in the regulation of mTORC1 activity. Proliferating cells tend to allocate substantial fraction of glucose to glycolytic ATP production followed by lactate generation accompanied by an increase in LDH activity. Although the role of LDH activity in mature SC was thoroughly documented, the role of LDH in immature SC remains obscure. The aim of this study was to analyze whether LDH activity is necessary to modulate mTORC1/p70S6K pathway and to regulate SC proliferation by FSH. SC obtained from 8-day old rats were maintained under basal conditions (B) or stimulated with FSH in the absence or presence of oxamate (OXA) -inhibitor of LDH activity. Lactate (Lac) production, phosphorylated (P)-mTORC1 and P-p70S6K levels by Western blot, and bromodeoxyuridine (BrdU) incorporation by immunocytochemistry were evaluated. Results are expressed as mean±SD of three independent experiments (different letters indicate statistically significant differences, P