INVESTIGADORES
CUNNINGHAM Monica Liliana
artículos
Título:
Embryo lipoproteins and yolk lipovitellin consumption during embryogenesis in Macrobrachium borellii (Crustacea: Palaemonidae)
Autor/es:
C.F. GARCIA; CUNNINGHAM M.; GARDA H.; HERAS H.
Revista:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART B, BIOCHEMISTRY & MOLECULAR BIOLOGY.
Editorial:
Elsevier
Referencias:
Año: 2008 vol. 151 p. 317 - 322
ISSN:
1096-4959
Resumen:
The prawn Macrobrachium borellii has lecithotrophic eggs with highly-abbreviated development. The major
yolk component is lipovitellin (LV), a lipoprotein with 30% lipids (by weight). LV consumption during
embryogenesis was followed by ELISA and Western blot analysis using an anti-LV polyclonal antibody. No
cross-reacting proteins were observed and LV-like lipoproteins were strongly recognized by the antibody in
hemolymph (vitellogenin), yolk (LV) and embryos (LVe), as determined by Western Blot analysis. LV
decreased significantly along development from 9.4 to 1.1 ìg/mg egg. Consumption rate of LV was slow in
early embryogenesis, followed by a rapid utilization in late embryonic stages. Significant LVe amounts were
still present at hatching. LV apolipoproteins were selectively degraded during embryo development, being
the highest molecular weight subunit the most affected. Comparison among in vitro, in vivo and theoretical
proteolysis suggested that trypsin may be involved in LV degradation during late embryogenesis. Embryo
lipoprotein (HDLe) synthesis was first detected at stage 6. HDLe shared the same density, MW and subunit
composition as adult hemolymph HDL1 and did not cross-react with LV-like lipoproteins. Though expressed
at low concentration, it fulfilled embryo needs for lipid transport among organs.
Macrobrachium borellii has lecithotrophic eggs with highly-abbreviated development. The major
yolk component is lipovitellin (LV), a lipoprotein with 30% lipids (by weight). LV consumption during
embryogenesis was followed by ELISA and Western blot analysis using an anti-LV polyclonal antibody. No
cross-reacting proteins were observed and LV-like lipoproteins were strongly recognized by the antibody in
hemolymph (vitellogenin), yolk (LV) and embryos (LVe), as determined by Western Blot analysis. LV
decreased significantly along development from 9.4 to 1.1 ìg/mg egg. Consumption rate of LV was slow in
early embryogenesis, followed by a rapid utilization in late embryonic stages. Significant LVe amounts were
still present at hatching. LV apolipoproteins were selectively degraded during embryo development, being
the highest molecular weight subunit the most affected. Comparison among in vitro, in vivo and theoretical
proteolysis suggested that trypsin may be involved in LV degradation during late embryogenesis. Embryo
lipoprotein (HDLe) synthesis was first detected at stage 6. HDLe shared the same density, MW and subunit
composition as adult hemolymph HDL1 and did not cross-react with LV-like lipoproteins. Though expressed
at low concentration, it fulfilled embryo needs for lipid transport among organs.
ficantly along development from 9.4 to 1.1 ìg/mg egg. Consumption rate of LV was slow in
early embryogenesis, followed by a rapid utilization in late embryonic stages. Significant LVe amounts were
still present at hatching. LV apolipoproteins were selectively degraded during embryo development, being
the highest molecular weight subunit the most affected. Comparison among in vitro, in vivo and theoretical
proteolysis suggested that trypsin may be involved in LV degradation during late embryogenesis. Embryo
lipoprotein (HDLe) synthesis was first detected at stage 6. HDLe shared the same density, MW and subunit
composition as adult hemolymph HDL1 and did not cross-react with LV-like lipoproteins. Though expressed
at low concentration, it fulfilled embryo needs for lipid transport among organs.
ficant LVe amounts were
still present at hatching. LV apolipoproteins were selectively degraded during embryo development, being
the highest molecular weight subunit the most affected. Comparison among in vitro, in vivo and theoretical
proteolysis suggested that trypsin may be involved in LV degradation during late embryogenesis. Embryo
lipoprotein (HDLe) synthesis was first detected at stage 6. HDLe shared the same density, MW and subunit
composition as adult hemolymph HDL1 and did not cross-react with LV-like lipoproteins. Though expressed
at low concentration, it fulfilled embryo needs for lipid transport among organs.
in vitro, in vivo and theoretical
proteolysis suggested that trypsin may be involved in LV degradation during late embryogenesis. Embryo
lipoprotein (HDLe) synthesis was first detected at stage 6. HDLe shared the same density, MW and subunit
composition as adult hemolymph HDL1 and did not cross-react with LV-like lipoproteins. Though expressed
at low concentration, it fulfilled embryo needs for lipid transport among organs.
first detected at stage 6. HDLe shared the same density, MW and subunit
composition as adult hemolymph HDL1 and did not cross-react with LV-like lipoproteins. Though expressed
at low concentration, it fulfilled embryo needs for lipid transport among organs.
1 and did not cross-react with LV-like lipoproteins. Though expressed
at low concentration, it fulfilled embryo needs for lipid transport among organs.filled embryo needs for lipid transport among organs.