INVESTIGADORES
PAGLINI Maria Gabriela
artículos
Título:
Evidence for the involvement of Tiam1 in axon formation
Autor/es:
KUNDA, P,; PAGLINI, G,; QUIROGA, S,; KOSIK, K; CÁCERES, A
Revista:
JOURNAL OF NEUROSCIENCE
Editorial:
Society for Neuroscience
Referencias:
Lugar: United States; Año: 2001 vol. 21 p. 2361 - 2371
ISSN:
0270-6474
Resumen:
In cultured neurons, axon formation is preceded by the appearance
in one of the multiple neurites of a large growth cone
containing a labile actin network and abundant dynamic microtubules.
The invasion-inducing T-lymphoma and metastasis 1
(Tiam1) protein that functions as a guanosine nucleotide exchange
factor for Rac1 localizes to this neurite and its growth
cone, where it associates with microtubules. Neurons overexpressing
Tiam1 extend several axon-like neurites, whereas suppression
of Tiam1 prevents axon formation, with most of the
cells failing to undergo changes in growth cone size and in
cytoskeletal organization typical of prospective axons. Cytochalasin
D reverts this effect leading to multiple axon formation
and penetration of microtubules within neuritic tips devoid
of actin filaments. Taken together, these results suggest that by
regulating growth cone actin organization and allowing microtubule
invasion within selected growth cones, Tiam1 promotes
axon formation and hence participates in neuronal polarization.