INVESTIGADORES
SERRA Esteban Carlos
artículos
Título:
Characterization of a Omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities
Autor/es:
GIRARDINI, JAVIER; AMIRANTE, ALEJANDRO; ZEMZOUMI, KHALID; ESTEBAN SERRA
Revista:
EUROPEAN JOURNAL OF BIOCHEMISTRY
Editorial:
Wiley
Referencias:
Año: 2002 vol. 269 p. 5512 - 5521
ISSN:
0014-2956
Resumen:
Glutathione S-transferases (EC 2.5.1.18) (GSTs), are a family of
multifunctional enzymes present in all living organisms whose main
function is the detoxification of electrophilic compounds. GSTs are
considered the most prominent detoxifying class II enzymes in helminths.
We describe here the characterization of novel dehydroascorbate
reductase and thiol transferase activities that reside in the human
parasite Schistosoma mansoni GSTx. Protein sequence analysis of this
parasite product showed lower identity to known GSTs. However,
phylogenic analysis placed SmGSTx among the recently described omega
class GSTs (GSTO1-1). We report here that SmGSTO protein is a 28-kDa
polypeptide, detected in all life stages of the parasite, being highly
expressed in adult worms. Like other omega
class GSTs, SmGSTO showed very low activity toward classical GSTs
substrates as 1-chloro-2,4-dinitrobenzene, and no binding affinity to
glutathione-agarose matrix but showed some biochemical characteristics
related with thioredoxins/glutaredoxins. Interestingly, SmGSTO was able
to bind S-hexyl glutathione matrix and displayed significant
glutathione-dependent dehydroascorbate reductase and thiol transferase
enzymatic activities.