INVESTIGADORES
SERRA Esteban Carlos
artículos
Título:
DNA polymerase Actity in Tomato Fruit Chromoplast
Autor/es:
ESTEBAN SERRA; CARRILLO, NESTOR
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 1990 vol. 173 p. 102 - 106
ISSN:
0014-5793
Resumen:
DNA polymerase activity was measured in chromoplasts of ripening tomato
fruits. Plastids isolated from young leaves or mature red fruits showed
similar DNA polymerase activities. The same enzyme species was present
in either chloroplasts or chromoplasts as judged by pH and temperature
profiles, sensitivities towards different inhibitors and relative
molecular mass (Mr 88 kDa). The activities analyzed showed the typical
behaviour of plastid-type polymerases. The results presented here
suggest that chromoplast maintain their DNA synthesis potential in fruit
tissue at chloroplast levels. Consequently, the sharp decrease of the
plastid chromosome transcription observed at the onset of fruit ripening
could not be due to limitations in the availability of template
molecules. Other mechanisms must be involved in the inhibition of
chromoplast RNA synthesis.