INVESTIGADORES
SERRA Esteban Carlos
artículos
Título:
Expression of SMNF-YA in Schistosoma mansoni
Autor/es:
ESTEBAN SERRA; LIU, JIN LI; CAPRON, ANDRE; ZEMZOUMI, KHALID; DISSOUS, COLETTE
Revista:
PARASITOLOGY
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Año: 1996 vol. 113 p. 457 - 464
ISSN:
0031-1820
Resumen:
The A subunit of NF-Y
nuclear factor from Schistosoma mansoni was expressed in E. coli fused
to a histidine tag and purified by affinity chromatography using a
Ni(2+)-Agarose matrix. Antibodies against the recombinant protein were
prepared and used for Western blot and immunolocalization. The presence
of SMNF-YA in all stages of the parasite life-cycle was determined by
RT-PCR and Western blot analysis. The immunolocalization of SMNF-YA
showed the presence of this factor in a parenchymal cell population of
cercariae and adult worms and in embryos within eggs. The expression of
SMNF-YA was demonstrated to decrease in maturating spermatozoites
whereas an accumulation of this factor was observed in the nucleus from
oocytes during their maturation processes.
