INVESTIGADORES
SERRA Esteban Carlos
artículos
Título:
Biomphalaria glabrata embryonic (Bge) cells express a protein with a domain homologous to the lectin domain of mammalian selectins
Autor/es:
ESTEBAN SERRA; DUCLERMONTIER, PASCAL; LARDANS, VINKA; TROLET, JAQUES; DISSOUS, COLETTE
Revista:
PARASITOLOGY RESEARCH
Editorial:
SPRINGER
Referencias:
Año: 1999 vol. 85 p. 481 - 486
ISSN:
0932-0113
Resumen:
We have cloned from Biomphalaria
glabrata, the intermediate host of the helminth parasite Schistosoma
mansoni, a 36-kDa apparent-molecular-mass molecule (BgSel) that shares
sequence identity with selectins of the cell-adhesion-molecule
superfamily. BgSel exhibited in its C-terminal part a putative C-type
lectin domain similar to the selectin lectin domain. Using antibodies to
the recombinant BgSel protein, we demonstrated the presence of BgSel in
snail hemocytes as well as in the cell line derived from B. glabrata
embryos (Bge). Anti-BgSel antibodies specifically recognized a 79-kDa
component in Bge-cell-secreted products that was supposed to represent
the native form of BgSel, as was confirmed after glycosidase treatment.
Lectins are known to be implicated in recognition mechanisms
participating in humoral and cellular immunity in molluscs. The possible
role of BgSel in the interaction between sporocysts and Bge cells,
particularly in the in vitro model of sporocyst development dependent on
Bge cell factors, remains to be determined.