INVESTIGADORES
BALDI Pablo Cesar
congresos y reuniones científicas
Título:
Mucosal levels of IgE+ and IgA+ cells and antibodies in protein deficient rats during Trichinella spiralis infection
Autor/es:
VILA, CC; SARACINO, MP; FALDUTO, GH; CALCAGNO, MA; PALLARO, A; BALDI, PC
Lugar:
Buenos Aires
Reunión:
Congreso; IUNS 21st International Congress of Nutrition; 2017
Institución organizadora:
International Union of Nutritional Sciences (IUNS)
Resumen:
Background and objectives: This study was aimed to analyze,in Trichinella spiralis-infected rats, the effect of protein deficiencyon antibody (Ab) levels and IgE+ and IgA+ cells counts in lung andintestine (relevant organs in protection against this pathogen).Methods: Weaning Wistar rats received a protein-deficientdiet (PD, 6.5% casein) or a control diet (C, 20% casein). After tendays, both groups were orally infected with T. spiralis muscle larvae(ML); non-infected rats served as controls.The organs were obtained at different days post-infection (p.i.).The numbers of IgA+ and IgE+ cells were determined by immunofluorescence.Total and specific Ab (against ML) were determinedby ELISA in tissue extracts. Data were analyzed using the two-wayANOVA.Results: Total IgE and IgG1 increased in C group from day 9p.i. in both organs. In lung also IgG2a increased from day 9 p.i. Inintestine IgA decreased from day 3 p.i. Although the same kineticswere observed in PD group for all isotypes, Ab levels were lowerthan in C group and did not increase significantly compared tonon-infected animals. Specific IgG1 was significantly lower in PDgroup in both organs.In lung IgE+ cells increased, compared to non-infected controls,in C group from day 3 p.i. (p<0.05) and in PD group fromday 33 p.i. (p<0.01). In intestine IgE+ cells increased in C groupfrom day 3 p.i. (p<0.001) and in PD group from day 6 p.i. (p<0.01).C group presented more IgE+ cells in both organs. No significantchanges were observed for IgA+ cells in any group.Conclusions: Protein deficiency seems to affect the mucosallevels of Ab and IgE+ cells during Trichinella spiralis infection.Given the important role of IgE in helminthiases, this may explainthe severe infection previously found in PD rats.
