Differential anthracycline sensitivity in two related human colon carcinoma cell lines expressing similar levels of P-glycoprotein

BOQUETE, A.L.; VARGAS ROIG, L.; LÓPEZ, G.A.; GUDE, R.; BINDA, M.M.; GONZÁLEZ, A.D.; CIOCCA, D.R.; BONFIL, R.D.

CANCER LETTERS

ELSEVIER IRELAND LTD

Año: 2001 vol. 165 p. 111 - 116

0304-3835

Chemosensitivity of the human colon carcinoma HCT-15 cell line to 4´-epidoxorubicin proved to be 100-fold higher than that of its variant HCT-15 EDR. Confocal scanning microscopy showed significant less drug accumulation in HCT-15 EDR. A 2-fold increase in hsp27 expression was found in HCT15 EDR, with no alteration in hsp70. The expression of the drug exporter Pgp was similar in both cell lines, despite the lower drug accumulation shown by HCT-15 EDR in respect to HCT-15. Other molecules implicated in the acquisition of enhanced chemoresistance or a more active Pgp variant present in HCT-15 EDR, could explain the phenomenon.