Optimization of hydrodinamical and adsorptive properties of Chromatographic Gigaporose Columns

MIRNA LORENA SANCHEZ; ACHILLI, ESTEFANIA; MARCELO FERNANDEZ LAHORE; MARIANO GRASSELLI

Newport

Conferencia; 17th International Conference on Biopartitioning and Purification; 2013

BPP

Modern biotechnology heavily depends on the availability of efficient processes, which have to generate competitive products in terms of quality and cost. Most commonly, the purification of bioproducts is based on chromatographic platforms. However, the low productivity imposed by diffusional limitations, the need for multiple separation steps, and the requirement of extensive solids separation, drives to a technological situation characterised by low global bioproduct yields [1]. In a previous work, we fabricated porous, opened, and reticulated polyurethane foams supporting a functional hydrogel layer. Preliminary studies showed that the adsorptive properties of these materials allow the reversibly binding of proteins by ion exchange mechanisms. Dynamic binding capacity tests (DBC) were performed employing lysozyme (HEWL) on a cation exchanger matrix. DBC experiments were carried out as a function column length, compression degree and flow velocity. Furthermore, bed hydrodynamics was studied via residence time experiments and correlated to the observed amount of protein captured. Experimental data indicated that optimum performance, as judged by Pe and Qdyn, was obtained when 6 column length, 3X compaction degree, and an 212 cm/h linear flow was utilized. The novel cation exchange material shows promise for the direct capture and purification of a range of biotechnology-derived products. [1] DePalma A. Genetic Engineering News 29 (2009) 8 M.L.S. thanks to CONICET and DAAD for the fellowship. M.G. is member of CONICET.