INVESTIGADORES
GRASSELLI Mariano
artículos
Título:
Preparation and characterisation of Cibacron Blue F3G-A poly(ethylene) hollow-fibre affinity membranes
Autor/es:
WOLMAN, FEDERICO J; MARIANO GRASSELLI; E SMOLKO, EDUARDO; CASCONE, O.
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2000 vol. 22 p. 1407 - 1411
ISSN:
0141-5492
Resumen:
Poly(ethylene) hollow-fibre membranes with immobilised Cibacron Blue F3G-A were obtained in four different ways from epoxy-activated fibres. Membranes with a maximum capacity of 26 mg lysozyme ml−1 and a dye density of 52 µmol ml−1 were obtained when ammonia was used to open the epoxy group before dye immobilisation. Pure water flux of the modified membranes at 1 bar pressure was 1.0 cm min−1, thus meaning only a reduction of 1.5-fold with regard to the unmodified membranes. The support-dye bond was stable as judged by the unmodified capacity of the membranes and the negligible amount of dye leaked after 520 h of exposure to 6 M urea in 0.5 M NaOH.
