INVESTIGADORES
GRASSELLI Mariano
artículos
Título:
Fungal pectic enzyme fractionation by dye affinity chromatography
Autor/es:
MARIANO GRASSELLI; CAMPERI, SILVIA A.; CASCONE, O.
Revista:
BIOTECHNOLOGY TECHNIQUES
Editorial:
SPRINGER
Referencias:
Año: 1996 vol. 10 p. 211 - 214
ISSN:
0951-208X
Resumen:
A dye affmity fractionation of a commercial pectic enzyme preparation is described. From nine immobilized triazinic dyes assayed, only Blue R-HE and Yellow 4R-HE were able to retain pectin lyase thus allowing the obtention of a fraction not producing methanol during fruit juice clarification. While pectinesterase did not bind to the chromatographic matrix, the retained pectin lyase could be quantitatively eluted with a salt gradient.
