INVESTIGADORES
GRASSELLI Mariano
artículos
Título:
Beaded matrices for affinity and ion exchange chromatography from cross-linked alginate
Autor/es:
MARIANO GRASSELLI; DIAZ, L.E.; CASCONE, O.
Revista:
BIOTECHNOLOGY TECHNIQUES
Editorial:
SPRINGER
Referencias:
Año: 1993 vol. 7 p. 707 - 712
ISSN:
0951-208X
Resumen:
Toobtainalawcost,beadedchromatographicmatrix,calciumalginatebeadswerecross-linked with epichlorohydtin, and calcium was removed by sodium citrate treatment. The cross-linking reaction fix obtaining stable beads was optimbi For pur&aticm of haemoglobin by ion exchange, crxos~liuked calcium-free alginate heads have a b of 60 mg/ml and a Kd of 0.02 mg/ml gel, while far af3?nity polygalacturonase pui&ation K&was 0.007 ml/g.
