ARSENICAL GRAFTED MEMBRANES FOR IMMOBILIZATION OF THIOREDOXIN-LIKE PROTEINS

CARBAJAL, M. L.; SOTO ESPINOZA, S.L.; VALDEZ, S.N.; POSKUS, E.; SMOLKO, E.E.; GRASSELLI, M.

REACTIVE & FUNCTIONAL POLYMERS

Elsevier

Año: 2009 vol. 69 p. 816 - 820

1381-5148

The modification of polymers by radiation grafting has been utilized for several decades. One of their distinctive properties, penetrability of gamma rays, allows to modify the internal surfaces of porous materials retaining its mechanical properties. In this work, we describe the cografting of glycidyl methacrylate and dimethyl acrylamide onto a macroporous polysulfone polymer. Aminophenyl arsenical compounds were covalently attached to the copolymer through epoxy ring add-on reactions following by a 2-mercaptoethanol activation. Thioredoxin and a thioredoxin-fusion protein were immobilized onto this surface and detected by specific antibody recognition. Preservation of native protein folding was confirmed by the detection of enzymatic activity of an unstable fusion protein. Immobilized fusion protein onto the modified material keeps the enzymatic activity for longer time (up to two weeks) against the free protein under the same storage conditions.