INVESTIGADORES
CIVELLO Pedro Marcos
artículos
Título:
Cloning of FaPAL6 gene from strawberry fruit and characterization of its expression and enzymatic activity in two cultivars with different anthocyanin accumulation.
Autor/es:
POMBO MA; MARTÍNEZ GA; CIVELLO, PM
Revista:
PLANT SCIENCE
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 181 p. 111 - 118
ISSN:
0168-9452
Resumen:
The accumulation of anthocyanin pigments is one of the most important
traits that turn strawberry fruit attractive to consumers. During
ripening, strawberry fruit color development is associated to
anthocyanin synthesis through the phenylpropanoid pathway. Phenylalanine
ammonia-lyase (PAL) is a key enzyme in this pathway, having a
determining role in strawberry fruit quality. In this work, we studied
the level of anthocyanins during fruit ripening of two cultivars that
differ in color development (Camarosa and Toyonoka). Toyonoka showed a
lower anthocyanin accumulation that was limited to external fruit
tissue, while Camarosa accumulated higher amount of anthocyanins in both
internal and external sections. In addition, we cloned a full-length
gene (FaPAL6) and analyzed its expression in different
strawberry plant tissues. The expression of this gene is fruit specific,
and increases during fruit ripening in both cultivars along with
anthocyanin accumulation. The mRNA level of FaPAL6 was higher
in Camarosa. PAL enzyme activity increased at similar rates in both
cultivars at early ripening stages, but at the end of ripening PAL
activity diminished in Toyonoka while it rose markedly in Camarosa. PAL
activity was higher in internal fruit tissue, showing no correlation
with anthocyanin level of the same section in both cultivars. The higher
FaPAL6 expression and activity detected in Camarosa could be
associated to the enhanced anthocyanin accumulation found in this
cultivar.