INVESTIGADORES
CIVELLO Pedro Marcos
artículos
Título:
UV-C treatment affects the expression and activity of defense genes in strawberry fruit (Fragaria x ananassa, Duch.).
Autor/es:
POMBO MA; ROSLI HG; MARTÍNEZ GA; CIVELLO, PM
Revista:
POSTHARVEST BIOLOGY AND TECHNOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 59 p. 94 - 102
ISSN:
0925-5214
Resumen:
UV-C treatment is a new and environment-friendly strategy to reduce
postharvest decay in several fruits. In this work, we studied the
induction of resistance to Botrytis cinerea in strawberry fruit, exposed
to a hormetic dose of UV-C. The results obtained showed that
pre-storage treatment of fruit with UV-C results in lower losses caused
by diseases and decay. Therefore, we analyzed the gene expression and
enzymatic activity of a set of strawberry genes that are related to
plant defense against pathogens. The expression of the genes and the
activity of assayed enzymes were modified in the treated fruit. The
expression and enzyme activity of phenylalanine ammonia lyase (PAL, EC:
4.3.1.24) increased over the level found in control after 4 and 24. h of
storage. The activity of β-1,3-glucanase (EC: 3.2.1.6) and peroxidase
(EC: 1.11.1.7) showed a biphasic pattern. Higher polyphenol oxidase
(PPO, EC: 1.10.3.1) activity was observed in treated fruit compared with
control from 10 to 48 h post-irradiation. Moreover, we studied the
expression of several strawberry PR proteins. We found an induction of
FaChi2-2 and FaChi3 immediately after the treatment, and the
corresponding chitinase (EC: 3.2.1.14) activity increased 10. h
post-treatment. Gene expression profile of β-1,3-glucanases was
different from those of chitinases. FaBG2-1 mRNA levels increased in the
treated fruit during the beginning of storage, but in the case of
FaBG2-3, irradiated fruit showed higher expression than the control
after 24 and 48 h of storage. Finally, FaPR1 gene expression was
stimulated by UV-C treatment at 4 and 24 h of storage. No changes in
FaOLP2 expression in fruit were found in response to irradiation.
Therefore, the reduction in strawberry fruit decay by UV-C treatment at
harvest could be related to the increase in the transcription and
activity of a set of enzymes and proteins involved in the defense
against pathogens. © 2010 Elsevier B.V.