Impaired function of dendritic cells deficient in angiotensin II type 1 receptors.

NAHMOD K; GENTILINI C; VERMEULEN M; UHAREK L; WANG Y; ZHANG J; SCHULTHEISS HP; GEFFNER J; WALTHER T

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS

AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS

Año: 2010 vol. 334 p. 854 - 862

0022-3565

Dendritic cells (DC) are highly specialized antigen-presenting cells with a unique ability to activate resting T lymphocytes and initiate primary immune responses. Angiotensin II (AII) is involved in key events of the inflammatory response. Because our previous work implicated an effect of AII on differentiation and function of murine and human DC, we investigated the impact of AII type 1 receptor (AT1) deficiency on the phenotypical and functional properties of mouse DC in vitro and in vivo. Bone marrow (BM) cells isolated from mice lacking AII subtype 1a receptor (AT1a), AII subtype 1b receptor (AT1b), or both receptor isoforms and control littermates [wild type (WT)] were cultured for 7 days in the presence of recombinant mouse granulocyte/macrophage colony-stimulating factor to generate myeloid DC in vitro. Generation of CD11c
cells was less efficient in both AT1a- and AT1b-deficient BM cells than in WT BM cell cultures. Moreover, DC generated from AT1-deficient progenitors showed lower levels of expression of major histocompatibility complex II (MHC-II) and CD11c (p 0.01) and a marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p progenitors showed lower levels of expression of major histocompatibility complex II (MHC-II) and CD11c (p 0.01) and a marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p BM cell cultures. Moreover, DC generated from AT1-deficient progenitors showed lower levels of expression of major histocompatibility complex II (MHC-II) and CD11c (p 0.01) and a marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p progenitors showed lower levels of expression of major histocompatibility complex II (MHC-II) and CD11c (p 0.01) and a marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p efficient in both AT1a- and AT1b-deficient BM cells than in WT BM cell cultures. Moreover, DC generated from AT1-deficient progenitors showed lower levels of expression of major histocompatibility complex II (MHC-II) and CD11c (p 0.01) and a marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p progenitors showed lower levels of expression of major histocompatibility complex II (MHC-II) and CD11c (p 0.01) and a marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p BM cell cultures. Moreover, DC generated from AT1-deficient progenitors showed lower levels of expression of major histocompatibility complex II (MHC-II) and CD11c (p 0.01) and a marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p progenitors showed lower levels of expression of major histocompatibility complex II (MHC-II) and CD11c (p 0.01) and a marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p marked reduction in their allostimulatory activity (p 0.01 or 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p 0.001). Although AT1-deficient DC released comparable levels of interleukin (IL)-10 and IL-12p70 to WT DC, they produced significantly lower levels of tumor necrosis factor
(TNF-
) (p of interleukin (IL)-10 and IL-12p70 to WT