INVESTIGADORES
VERMEULEN Elba Monica
artículos
Título:
Sphingosylphosphorylcholine activates dendritic cells, stimulating the production
Autor/es:
CEBALLOS A., SABATTÉ J., NAHMOD K., AMARAL MM., MARTÍNEZ D., SALAMONE G., VERMEULEN M., MAGGINI J., SALOMÓN H., GEFFNER J
Revista:
IMMUNOLOGY
Editorial:
British Society for Immunology
Referencias:
Lugar: London; Año: 2007 vol. 121 p. 328 - 336
ISSN:
0019-2805
Resumen:
Compared with other lysophospholipid mediators such as sphingosine-1-
phosphate and lysophosphatidic acid, little is known about the physiological
significance of the related bioactive lysosphingolipid sphingosylphosphorylcholine
(SPC), which is present in high-density lipoprotein particles. The
present study was undertaken to evaluate the effect of SPC on human
immature dendritic cells (DCs). Reverse transcriptionpolymerase chain
reaction and flow cytometry assays revealed that DCs express two putative
receptors for SPC, ovarian cancer G-protein-coupled receptor 1 and G-protein-
coupled receptor 4. Exposure to SPC induced a rapid and transient
increase in intracellular free calcium concentrations but did not stimulate
endocytosis or chemotaxis of DCs. SPC increased the expression of HLADR,
CD86 and CD83 and improved the T-cell priming ability of DCs, as
well as the ability of DCs to stimulate the production of interferon-c by allogeneic
peripheral blood mononuclear cells during the mixed lymphocyte
reaction. Consistent with these results, we also observed that SPC stimulated
the production of interleukin (IL)-12 and IL-18 by DCs. Taken together,
our results support the notion that the accumulation of SPC in peripheral
tissues during the course of inflammatory processes may favour the
development of T helper type 1 immunity.c by allogeneic
peripheral blood mononuclear cells during the mixed lymphocyte
reaction. Consistent with these results, we also observed that SPC stimulated
the production of interleukin (IL)-12 and IL-18 by DCs. Taken together,
our results support the notion that the accumulation of SPC in peripheral
tissues during the course of inflammatory processes may favour the
development of T helper type 1 immunity.