INVESTIGADORES
FANARA Juan Jose
congresos y reuniones científicas
Título:
Candidate genes and SNPs for body size related traits in Drosophila melanogaster
Autor/es:
ORTIZ, V; FANARA JJ; CARREIRA, VP
Lugar:
Raleigh
Reunión:
Congreso; Evolution meeting; 2014
Institución organizadora:
Society for the Study of Evolution
Resumen:
Candidate
genes and SNPs for body size related traits in Drosophila melanogaster
Victoria Ortiz, Universidad de Buenos Aires,
vicrotas@gmail.com
Juan José Fanara, Universidad de Buenos Aires, Argentina, jjfanara@ege.fcen.uba.ar
Valeria Carreira,
Universidad de Buenos Aires, vpcarreira@ege.fcen.uba.ar
Body size
is a complex character which is associated to many important fitness related
traits. In order to study its genetic basis, we analyzed face width (FW),
thorax length (TL) and wing length (WL) in 40 lines of the Drosophila Genetic
Reference Panel (i.e. isogenic lines of D. melanogaster derived from a natural
population whose nuclear genomes were completely sequenced). We identified 19,
20 and 114 Single Nucleotide Polimorphisms (SNPs) associated to variation in
FW, TL and WL respectively, most of which are located in intronic and
intergenic regions. These candidate SNPs are associated to 18, 15 and 89 genes
for FW, TL and WL respectively. Some SNPs are related to more than one
candidate gene as well as multiple SNPs affect a single gene in many cases. We
found only two genes, A2bp1 and axo, affecting two traits simultaneously (i.e.
TL and WL) and no gene affecting all of them at the same time. Furthermore,
SNPs affecting both traits simultaneously were different for each gene. These
results suggest a low level of pleiotropy regarding the studied characters. The
distribution of these genes through the genome departed from a random one as we
found far less candidate genes located in the 3R chromosome than expected.
Finally, a gene ontology analysis associated 93 annotated genes with 118 terms
of Biological Process including anatomical structure morphogenesis, organ
development, locomotion, tissue development, imaginal disc morphogenesis and
imaginal disc-derived appendage morphogenesis. Most candidate SNPs associated
to TL and WL showed sex-specific results although an important number of SNPs
were associated to WL variation in both sexes simultaneously. Several of these
SNPs are associated to genes as fruitless, which have been related to
inter-male aggressive behavior. On the other side, most SNPs associated to FW
were related to the SNP by sex interaction, although some of them showed
sex-specific results. One of the two genes related to variation in males´s FW,
Gr39a, has been associated to courtship behavior in males. Finally, our results
confirmed five candidate genes which have been related to different
morphological traits using other type of isogenic lines (e.g. CG9171, CG42684,
bif, bol and ed), many of which have been associated to inter-male aggressive
behavior. These results greatly contribute to the knowledge regarding the
genetic basis of body size traits as well as they raise interesting questions
concerning its relationship with characters related to sexual behavior.