INVESTIGADORES
GOIN Juan Carlos
congresos y reuniones científicas
Título:
Antitumor activity of a Salmonella Typhi vaccine strain in a mouse model of T-cell lymphoma
Autor/es:
VENDRELL, A.; TESONE, A.; DE TORO, J.; HERSCHLIK, L.; GRAVISACO, M.J.; GOIN J.C.; LAROTONDA, G.; MONGINI, C.; WALDNER, C.
Lugar:
Milan
Reunión:
Congreso; 15th International Congress of Immunology; 2013
Institución organizadora:
International Union of Immunological Societies (IUIS)
Resumen:
Previously we demonstrated the therapeutic efficacy of an attenuated vaccine strain of SalmonellaTyphi as a cancer immunotherapy in a mouse model of subcutaneous T cell lymphoma. EL4
tumor-bearing mice treated with Salmonella Typhi CVD 915, by injection
into the tumor and in the area of the draining lymph nodes, showed
reduced tumor growth and increased survival. In this work, we further
studied the therapeutic efficacy and the antitumor effector mechanisms
promoted by this bacterium. Our results indicate that the treatment with
Salmonella promoted a reduction in the mitotic index of the tumors (p
<0.05) and delayed the development of palpable metastases in the
lymph nodes (p <0.001). Furthermore, the administration of Salmonella
in tumor-bearing mice promoted leukocyte infiltration, mainly
neutrophils (p <0.01) and decreased IL- 10 levels in the tumor
microenvironment (p <0.05), and also reduced LTreg frequency in tumor
draining lymph nodes. Under these conditions, Salmonella remained
viable within the tumor, and tumor- infiltrating neutrophils released
TNF-alpha and expressed bacterial antigens. Finally, Salmonella was found to
bind to tumor cells and exert oncolytic activity upon direct contact
with the tumor cell. These results demonstrate the efficacy of a vaccine
strain of Salmonella Typhi as an oncolytic and immunotherapeutic agent
against a highly malignant tumor