Tubulin Aggregates Induced by Ni2+ Present Microtubular Characteristics

BERON, W; LOPEZ, L; BERTINI, F

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 1995 vol. 19 p. 183 - 188

0327-9545

Rat brain tubulin in a proper buffered solution became insoluble in the presente of 10 mM NiCl,, and sedimented at centrifugal forces as low as 500 x g for 30 min. Both nickel-sedirnented and microtubular tubulin conserved 65% of colchicine binding activity after 25 days of storage at -20°C. However in brain cytosol, only 9% of the initial binding activity was conserved. The electrophoretic mobility of tubulin recovered from aggregates also remained unaltered. Therefore the aggregates formed with Ni2+ share important physicochemical properties with microtubules.