FKBP51 protein increase cell proliferation in HEK293 cells

BENDER T, DE LEO S, GALIGNIANA MD, ERLEJMAN A

Congreso; LXIV Reunión Anual de SAIC; 2019

SAIC

The FK506-binding protein 51 (FKBP51) was first described as regulatory factor of steroid receptors. Currently, it is regarded that it also regulates diverse transcription factors (TFs). Previously, we demonstrated that FKBP51 modulates the transcriptional activity of NF-B, AP-1 and E2F. Because, these TFs participate dynamically in cell proliferation, ours aim was to investigate the effect of FKBP51 on cell proliferation. HEK293 cells were transfected with pCI-Neo or pCI-Neo-hFKBP51 expression plasmids. After one month of selection the stable transfected clones were selected. HEK51 cells stably overexpress FKBP51 protein, in the same way, HEKpCI-Neo were selected containing the empty vector. Cell proliferation (viable cell count with Trypan Blue dye), proliferation index (flow cytometric analysis of cell division using dilution of CFSE) and cell cycle distribution (flow cytometry analysis of propidium iodide DNA stained) were evaluated. A Human Gene Expression Microarrays (SurePrint G3 Human GE v2 8x60K, Agilent) were performed for HEK51 vs. HEKpCI-Neo.