INVESTIGADORES
PICOLLO Maria Ines
congresos y reuniones científicas
Título:
Almost fixation in knockdown resistance alleles to pyrethroids in head louse (Pediculus humanus capitis) populations from Argentina
Autor/es:
TOLOZA A; ASCUNCE M; REED D.; PICOLLO M.I.
Lugar:
Park City, Utah
Reunión:
Congreso; 5º International Conference on Phthiraptera 2014; 2014
Institución organizadora:
University of Utah
Resumen:
In Argentina, the overuse of products containing permethrin (a common
used synthetic pyrethroid) in the last three decades has led to the development
of resistant louse populations. Pyrethroid insecticides act on the nervous
system affecting voltage-sensitive sodium channels (VSSC). Two point mutations
at the corresponding amino acid sequence positions T917I and L920F in the
voltage-gated sodium channel gene are responsible for mainly contributing to
knockdown resistance (kdr). The
management of pyrethroid resistance requires either early detection or the
characterization of the mechanisms involved in head louse populations that are
essential to perform optimal control strategies. In the present study, we
estimated the geographical distribution of kdr
alleles in 154 head lice from six geographic regions throughout Argentina. Pyrethroid
resistance kdr alleles were found in
high frequencies ranging from 67 to 100%, reaching almost fixation. Of these,
131(85.1%) were homozygous resistant, 13 (8.4%) were homozygous susceptible,
and 10 (6.5%) were heterozygous. Exact tests for Hardy-Weinberg equilibrium for
each location showed that genotype frequencies differed significantly from
expectation in 4 of the 6 sites studied (67%). These results show that
pyrethroid resistance is well established and has evolved rapidly among head
louse populations from Argentina.