Identification of the apomixis controlling region (ACR) in the genome of the diploid sexual cytotype of Paspalum notatum.

SPOTO, NICOLÁS; PODIO, MARICEL; GRISOLIA, MAURICIO; ROHR, CRISTIAN; PESSINO, SILVINA; ORTIZ, JUAN PABLO

Mendoza

Congreso; XVII Congreso Latinoamericano de Genética.; 2019

Sociedad Argentina de Genética

Apomixis is an asexual mode of reproduction by seeds associated with polyploidy. It is anadvantageous trait for agriculture because it guarantees the maintenance of heterosis. P. notatumforms a multiploid species with diploid sexual self-sterile and polyploidaposporous apomicticself-fertile cytotypes. Apomixis in the tetraploid race is controlled by a single chromosomal region(ACR) that showed segregation distortion, lack of recombination and synteny with segmentsof rice chromosomes 2 and 12. Apospory (one of the apomixis components) was observed indiploid genotypes, although with low expressivity. The objectives of this work were to localize thehomologous region/s of the ACR in the diploid genome and identify putative candidates. A draftassembly of the diploid genome, generated by a combination of Illumina® and Oxford Nanopore®reads, was used as a reference. Twenty ACR-specific sequences from the tetraploid genotype Q4117 were blasted (BLASTN, score>250, E value e