Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain.

MORETTA R; RUYBAL P; MESPLET M; PETRIGH R; NUÑEZ P,; GIL G.; WILCOWSKY S; GARBOSSA G.; FARBER M

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES.

Annals of the New York Academy of Sciences

Lugar: New York; Año: 2008 vol. 1149 p. 111 - 113

0077-8923

In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections.