INVESTIGADORES
FARBER Marisa Diana
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Título:
Babesia bovis merozoite surface protein-2c (MSA-2c) contains highly immunogenic, conserved B-cellepitopes that elicit neutralization-sensitive antibodies in cattle
Autor/es:
S.E. WILKOWSKY; M. FARBER; I. ECHAIDE; S.TORIONI DE ECHAIDE; P.I. ZAMORANO; M. DOMINGUEZ; C.E. SUAREZ; M. FLORIN-CHRISTENSEN
Revista:
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2003 vol. 127 p. 133 - 141
ISSN:
0166-6851
Resumen:
ThesearchforvaccinecandidatesagainstbovinebabesiosiscausedbyBabesiabovisisgreatlyfocusedontheidentificationofmero-zoitesurface-exposedantigensthatarewidelyconserved,functionallyrelevantandimmunodominantincattleprotectedagainstB.bovisinfections.Wehaverecentlyidentifiedmsa-2c,amemberoftheB.bovisvariablemerozoitesurfaceantigen(VMSA)genefamily,whichincontrasttoothermembers,appearstobehighlyconservedamonggeographicallydistantB.bovisstrains.Inthisstudy,wefurtherinvestigatedthepotentialofthemsa-2cgeneproductasdiagnosticandvaccinecandidateforbovinebabesiosis.RT-PCRstudiesdemon-stratedthatMSA-2cistranscribedinmerozoitesoftheArgentineR1Astrain.Inaddition,antibodiesagainstR1ArecombinantMSA-2creactedinimmunoblotswithasingleproteinofapproximately30kDainB.bovismerozoiteextractsfrombothR1AandAustralian“S”strains,demonstratingtranslationofthisproteininthesetwostrainsandconservationofB-cellepitopesbetweenthem.TheseantibodiesreactedwiththecellsurfaceofR1Amerozoitesinfixedimmunofluorescenceassays,indicatingthesurfacelocalizationofMSA-2c.Thislocalizationwasconfirmedbyliveimmunofluorescencestudiesintwodifferentstrains,R1AandS2P.TheseresultsalsodemonstratetheconservationofMSA-2csurface-exposedB-cellepitopesbetweenthesetwostrains.SerafromcattleeithernaturallyorexperimentallyinfectedwithArgentinestrainsofB.bovisspecificallyrecognizedrMSA-2cinimmunoblots,reinforcingtheideathatB-cellepitopesinrMSA-2carewidelyconservedamongfieldstrainsofB.bovis.Furthermore,ourresultsshowthattheseB-cellepitopesarehighlyimmunogenic,suggestingthatMSA-2cmaybeausefuldiagnostictoolforthedetectionofbovinebabesiosisbyB.bovis.ExperimentalvaccinationoffivebovineswithrMSA-2cresultedinelicitationofhighspecificanti-rMSA-2cIgGtiters,withsimilaramountsofIgG1andIgG2produced.Importantly,bovineanti-rMSA-2cantibodieswereabletoneutralizeinvitrobovineerythrocyteinvasionbyR1AmerozoitessuggestingasignificantfunctionalroleforMSA-2c.TakentogethertheseresultspostulateMSA-2casacandidateforthedevelopmentofnoveltoolsforimprovedcontrolofbovinebabesiosis
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