Encapsulation of Resveratrol in nanovesicles derived from renewable sources

NOELIA MACHADO; PABLO GARCÍA MANRIQUE; GEMMA GUTIÉRREZ; MARÍA DEL CARMEN PAZOS; MARIANA A. FERNÁNDEZ

Mendoza

Simposio; Resveratrol 2017-Regional Meeting; 2017

UNCuyo

Resveratrol (RSV) is a natural polyphenol mainly found in the seeds andskin of grapes, and red wine. It exists in two isomeric structures, but onlytrans-RSV has potential health benefits in combating diseases, such ascancer, diabetes, neurodegeneration, cardiovascular disorders,inflammation, and other age-related pathologies.However, this great RSV potential is hindered by its poorpharmacokinetic properties, such as low aqueous solubility, lowphotostability, short biological half-life, and rapid metabolism andelimination. To overcome these limitations, encapsulation of RSV seemsto be a good alternative for using this ingredient in food, pharmaceuticaland cosmetic industries.Niosomes are vesicles of nanometric size formed by the self-assembly ofnon-ionic surfactants. As drug delivery systems, they offer importantadvantages over other encapsulation technologies, such as their low cost,high stability, and biocompatibility.In our study, niosomes were formulated using Tween 80 and Span 80.Both are food grade surfactants derived from sorbitol (sugar) and oleicacid (fatty acid). Dodecanol (fatty alcohol) was used as membranestabilizer.Niosomes were prepared by the thin film hydration method. Theniosomal formulations were evaluated measuring their particle size andencapsulation efficiency (EE). In order to determine EE, free andencapsulated RSV were separated by means of dialysis and gelchromatography.The niosomes mean size was ~227 nm and no considerable differenceswere found due to the presence of dodecanol. However, EE for niosomesformulated with dodecanol were higher than those achieved in itsabsence. On the other hand, when gel chromatography was used as freeRSV separation method, EE were greater than those obtained usingdialysis.The best formulation enhanced 4 times the RSV aqueous solubility. Allthese results indicated that our niosomes are able to encapsulate RSV andcould control RSV release when these nanovesicles are used in food,cosmetic or pharmaceutical applications.