Comparación de los niveles de carga proviral y expresión de ARNm de citoquinas entre células mononucleares de sangre periférica y células somáticas de la leche de animales infectados con BLV

DOLCINI GUILLERMINA LAURA; LADERA MARLA ELIANA; NIETO FARIAS, MARIA VICTORIA; VATER, ADRIAN ALEJANDRO; CERIANI MARIA CAROLINA

Congreso; I Congreso Argentino Microbiologia Veterinaria; 2021

Asociacion Argentina Microbiologia

The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle