Perinatal exposure to bisphenol a (BPA) alters kiss1 expression and impairs neuroendocrine mechanisms regulating food intake in adult male rats.

STOKER C; ANDREOLI MF; ROSSETTI MF; KASS L; BOSQUIAZZO VL; RAMOS JG

Workshop; IWNE 2015; 2015

Kisspeptin (kiss1) is a hypothalamic neuropeptide that drives fertility by stimulating GnRH secretion. Evidences show that kiss1 could be the link between energy balance and reproductive function. Bisphenol A (BPA) is a compound used in the polymerization of polycarbonate plastics and has activity as endocrine disruptor. Our objective was to determine the influence of perinatal exposure to a dose considered safe of BPA on body weight, metabolic parameters and hypothalamic signals that regulate food intake, both in adult males fed with regular diet or a high fat diet.Male rats were exposed to 50 g/kg/day of BPA or vehicle (0.002 % ethanol) from day 9 of gestation to weaning in the drinking water. Since weaning, males were fed with control diet (CD) or high fat diet (HFD) for 20 weeks. We evaluated: energy intake, body and fat pad weights, glucose tolerance test (GTT) and hypothalamic mRNA expression of the neuropeptides: Kiss1, POMC, CART, AGRP, NPY; the receptors: InsR, ob-Rb, ER, ER and the relative activity of its promoters (OS, O, OT, and E1). Serum insulin, testosterone and estradiol were also assessed.BPA exposure induces an increase in body and fat pad weights as a consequence of a higher energy intake (p