INVESTIGADORES
PERELLO Analia Edith
artículos
Título:
Isozyme polymorphisms within Pyrenophora tritici repentis population inArgentina
Autor/es:
MORENO, V; TACALITTI, MS; CASTRO, A.; PERELLÓ, A.
Revista:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Editorial:
Springler
Referencias:
Año: 2008 vol. 24 p. 849 - 860
ISSN:
0959-3993
Resumen:
Tan spot caused by Pyrenophora tritici-repentis
is an economically important disease of wheat (Triticum
aestivum L.) in Argentina. The success of local breeding
programs for resistance to the disease depends to a large
extent on the genetic variation within the pathogen population.
To assay this variability, 155 isolates of P. triticirepentis
obtained from leaves of wheat and Festuca sp. of
different localities were assayed and compared for isozyme
polymorphisms. Isozyme analysis was performed with
polyacrilamide gel electrophoresis in the systems estearase,
phosphatase and peroxidase. Variation in the number and
relative mobility of the bands by each system was detected
in this collection of isolates. Intraspecific polymorphisms
were identified in the three systems assayed. The cluster
analysis based in bands patterns defined 86 electromorph
types among the 155 isolates evaluated according with the
Jaccard?s coefficient (CCC = 095). The isozymes patterns
were not correlated with the geographic and/or wheat
cultivars origin of the P. tritici-repentis isolates.
Keywords Electrophoretic _ Isozyme _ Polymorphisms _
Pyrenophora tritici-repentis _ Variability _ Wheat