INVESTIGADORES
IBAÑEZ Gabriela Alejandra
artículos
Título:
Determination of triamterene in pharmaceutical formulations and of triamterene and its main metabolite hydroxytriamterene sulfate in urine using solid-phase and aqueous solution luminescence
Autor/es:
G.A. IBAÑEZ; G.M. ESCANDAR; A. ESPINOSA MANSILLA; A. MUÑOZ DE LA PEÑA
Revista:
ANALYTICA CHIMICA ACTA
Editorial:
Elsevier
Referencias:
Lugar: Asterdam; Año: 2005 vol. 538 p. 77 - 84
ISSN:
0003-2670
Resumen:
Abstract
Solid-phase extraction (SPE) (reversed-phase and mixed-mode) and nylon membranes were investigated as solid matrices for obtaining
fluorescence signals from adsorbed triamterene (TA). Although a significant emission was found in all investigated surfaces, solid-phase
extraction systems yielded the best results. The analytical figures of merit obtained under the best experimental conditions, using nonpolar/
strong cation mixed phase MP1 discs and 5ml of extracting sample, were: linear calibration range from 1.7 to 21 ng ml−1 (the lowest value
corresponds to the quantitation limit), relative standard deviation, 1.3% (n = 5) at a level of 12.7 ng ml−1, and limit of detection, 0.6 ng ml−1
corresponds to the quantitation limit), relative standard deviation, 1.3% (n = 5) at a level of 12.7 ng ml−1, and limit of detection, 0.6 ng ml−1
−1 (the lowest value
corresponds to the quantitation limit), relative standard deviation, 1.3% (n = 5) at a level of 12.7 ng ml−1, and limit of detection, 0.6 ng ml−1n = 5) at a level of 12.7 ng ml−1, and limit of detection, 0.6 ng ml−1
(calculated according to Clayton?s definition, taking into account false positive and false negative errors). An approach for the spectrofluorimetric
determination of triamterene in pharmaceutical formulations was successfully applied. In addition, a simple and rapid method for
the separation and fluorimetric determination of triamterene and its main metabolite (hydroxytriamterene sulfate) in urine was developed and
rendered very satisfactory results, requiring in this case the use of reversed-phase C18 solid-phase extraction.