Evaluation of a blocking ELISA using an urease conjugate for detection of antibodies to Pseudorabies virus

ECHEVERRIA MG; NOSETTO EO; ETCHEVERRIAGARAY ME

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION

AMER ASSOC VETERINARY LABORATORY DIAGNOSTICIANS INC

Año: 2000 vol. 12 p. 266 - 268

1040-6387

Abstract. A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions. A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.