Study of enantioseparation of β-blockers using amylose tris(3-chloro-5-methylphenylcarbamate) as chiral stationary phase under polar-organic, reversed-phase and hydrophilic interaction liquid chromatography conditions

MERINO, MATÍAS E. DÍAZ; LANCIONI, CARLINA; PADRÓ, JUAN M.; CASTELLS, CECILIA B.

JOURNAL OF CHROMATOGRAPHY - A

ELSEVIER SCIENCE BV

Año: 2020 vol. 1634

0021-9673

In this study, we describe the experimental variables influencing enantioseparation of twelve β-blockers when analyzed under polar-organic, reversed-phase and hydrophilic interaction liquid chromatography conditions on a column with immobilized amylose tris (3-chloro-5-methylphenylcarbamate) as chiral stationary phase. Regarding polar-organic mode, two component mobile phases consisting of methanol, ethanol or acetonitrile with the addition of basic additives such as diethylamine, triethylamine, mono- ethanolamine, ethylendiamine or trifluoroacetic acid/diethylamine mixture were evaluated. Studies of re- tention at different temperatures were also performed. In reversed-phase mode, mixtures consisting of methanol or acetonitrile with either aqueous boric acid-borate buffer or sodium hydrogen carbonate- carbonate buffer solutions were compared aiming to study the influence of organic modifier as well as buffer type and pH on resolution. In addition, a systematic evaluation of the retention factors of β- blockers enantiomers in hydro-organic eluents containing acetonitrile in presence of diethylamine as additive was carried out by increasing progressively the water content, in order to check for reten- tion dependencies indicative of the interplay of both hydrophilic interaction liquid chromatography and reversed-phase modes.