?Enantioselective gas chromatography with functionalized cyclodextrins as chiral selectors. Fundamentals of the measurement of absolute association constants using capillary columns

MENESTRINA, F.; RONCO, N.; CASTELLS, C. B.

JOURNAL OF CHROMATOGRAPHY - A

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 1467 p. 482 - 489

0021-9673

Chiral capillary GC columns containing different amounts of octakis(6-O-tertbutyldimethylsilyl-2,3-di-O-acetil)-γ-cyclodextrin as chiral selector dissolved in a polymeric matrix were constructed with the aim of determining enantiomeric association constants between a group of well resolved chiral N-trifluoroacetyl amino acid methyl esters and this specific selector at different temperatures. The most relevant sources of uncertainties in the experimental data (hold-up and retention times, and column phase ratios at each temperature) were assessed.These cyclodextrin-based columns are known to enantioseparate a wide variety of chemicalcompounds, thus, the measurement of the absolute enantioselective constants of a group ofsolutes with this selector can be useful for systematic studies aimed to a general understanding about how these selectors work. These absolute association constants were estimated from data collected from very simplified experimental systems, and by using the fundamental gas-liquid chromatography equations.